# Transcriptomic response of Emiliania huxleyi to HHQ # PI: Kristen Whalen (Haverford College) # Co-PI: Elizabeth Harvey (Skidaway Institute of Oceanography) # Version date: 17-July-2019 ========================= series_accession biosample_accession biosample_link sample_name organism tax_ID strain sample_type biomaterial_provider env_biome samp_size temp light_level_umol_m2_s light_dark_hr media collection_date geo_loc_name treatment time_elapsed bio_replicate ------------------------- GSE131846 SAMN11874448 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874448 Parent culture replicate 1, used for inoculation Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 20-Jun-18 USA: Haverford College none 0 hr 1 GSE131846 SAMN11874483 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874483 Parent culture replicate 2, used for inoculation Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 20-Jun-18 USA: Haverford College none 0 hr 2 GSE131846 SAMN11874482 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874482 Parent culture replicate 3, used for inoculation Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 20-Jun-18 USA: Haverford College none 0 hr 3 GSE131846 SAMN11874481 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874481 Parent culture replicate 4, used for inoculation Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 20-Jun-18 USA: Haverford College none 0 hr 4 GSE131846 SAMN11874480 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874480 Vehicle control replicate 1, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College DMSO 24 hr 1 GSE131846 SAMN11874479 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874479 Vehicle control replicate 2, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College DMSO 24 hr 2 GSE131846 SAMN11874478 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874478 Vehicle control replicate 3, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College DMSO 24 hr 3 GSE131846 SAMN11874477 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874477 Vehicle control replicate 4, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College DMSO 24 hr 4 GSE131846 SAMN11874476 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874476 1 ng/ml HHQ replicate 1, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 1 ng/ml HHQ 24 hr 1 GSE131846 SAMN11874475 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874475 1 ng/ml HHQ replicate 2, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 1 ng/ml HHQ 24 hr 2 GSE131846 SAMN11874474 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874474 1 ng/ml HHQ replicate 3, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 1 ng/ml HHQ 24 hr 3 GSE131846 SAMN11874473 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874473 1 ng/ml HHQ replicate 4, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 1 ng/ml HHQ 24 hr 4 GSE131846 SAMN11874472 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874472 10 ng/ml HHQ replicate 1, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 10 ng/ml HHQ 24 hr 1 GSE131846 SAMN11874471 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874471 10 ng/ml HHQ replicate 2, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 10 ng/ml HHQ 24 hr 2 GSE131846 SAMN11874470 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874470 10 ng/ml HHQ replicate 3, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 10 ng/ml HHQ 24 hr 3 GSE131846 SAMN11874469 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874469 10 ng/ml HHQ replicate 4, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 10 ng/ml HHQ 24 hr 4 GSE131846 SAMN11874468 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874468 100 ng/ml HHQ replicate 1, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 100 ng/ml HHQ 24 hr 1 GSE131846 SAMN11874467 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874467 100 ng/ml HHQ replicate 2, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 100 ng/ml HHQ 24 hr 2 GSE131846 SAMN11874466 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874466 100 ng/ml HHQ replicate 3, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 100 ng/ml HHQ 24 hr 3 GSE131846 SAMN11874465 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874465 100 ng/ml HHQ replicate 4, 24 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 21-Jun-18 USA: Haverford College 100 ng/ml HHQ 24 hr 4 GSE131846 SAMN11874464 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874464 Vehicle control replicate 1, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College DMSO 72 hr 1 GSE131846 SAMN11874463 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874463 Vehicle control replicate 2, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College DMSO 72 hr 2 GSE131846 SAMN11874462 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874462 Vehicle control replicate 3, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College DMSO 72 hr 3 GSE131846 SAMN11874461 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874461 Vehicle control replicate 4, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College DMSO 72 hr 4 GSE131846 SAMN11874460 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874460 1 ng/ml HHQ replicate 1, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 1 ng/ml HHQ 72 hr 1 GSE131846 SAMN11874459 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874459 1 ng/ml HHQ replicate 2, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 1 ng/ml HHQ 72 hr 2 GSE131846 SAMN11874458 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874458 1 ng/ml HHQ replicate 3, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 1 ng/ml HHQ 72 hr 3 GSE131846 SAMN11874457 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874457 1 ng/ml HHQ replicate 4, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 1 ng/ml HHQ 72 hr 4 GSE131846 SAMN11874456 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874456 10 ng/ml HHQ replicate 1, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 10 ng/ml HHQ 72 hr 1 GSE131846 SAMN11874455 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874455 10 ng/ml HHQ replicate 2, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 10 ng/ml HHQ 72 hr 2 GSE131846 SAMN11874454 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874454 10 ng/ml HHQ replicate 3, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 10 ng/ml HHQ 72 hr 3 GSE131846 SAMN11874453 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874453 10 ng/ml HHQ replicate 4, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 10 ng/ml HHQ 72 hr 4 GSE131846 SAMN11874452 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874452 100 ng/ml HHQ replicate 1, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 100 ng/ml HHQ 72 hr 1 GSE131846 SAMN11874451 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874451 100 ng/ml HHQ replicate 2, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 100 ng/ml HHQ 72 hr 2 GSE131846 SAMN11874450 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874450 100 ng/ml HHQ replicate 3, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 100 ng/ml HHQ 72 hr 3 GSE131846 SAMN11874449 https://www.ncbi.nlm.nih.gov/biosample/SAMN11874449 100 ng/ml HHQ replicate 4, 72 hours after treatment Emiliania huxleyi 2903 CCMP2090 cell culture NCMA (https://ncma.bigelow.org) coastal temperate ocean ca. 5 x 10^7 cells 17.5 - 17.8 80 +/- 5 14:10 f/2 medium (without silicate) made with natural seawater base collected from Corson's Inlet State Park, NJ, USA 23-Jun-18 USA: Haverford College 100 ng/ml HHQ 72 hr 4